Understanding functional implication of β-casein gene variants in four cattle breeds characterized using AmpliSeq approach

@article{Patel2020UnderstandingFI,
  title={Understanding functional implication of $\beta$-casein gene variants in four cattle breeds characterized using AmpliSeq approach},
  author={Shiven B. Patel and T. Shah and Pritesh H. Sabara and D. Bhatia and K. Panchal and J. Italiya and P. Koringa and D. Rank},
  journal={3 Biotech},
  year={2020},
  volume={10}
}
Here, we designed a custom panel targeting whole β-casein gene SNPs of zebu and taurine cattle breeds to identify variants and applicability in dairy cattle genotyping. We sequenced two libraries consisting of different pools of primer sets from 95 individuals on the Illumina MiSeq. Consequently, over 92% target regions were amplified and 71 SNPs were available after quality filtering. Only three intronic variants were novel while majority of the identified variants were catalogued in dbSNP as… Expand
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