Understanding discrimination by the ribosome: stability testing and groove measurement of codon-anticodon pairs.

@article{Sanbonmatsu2003UnderstandingDB,
  title={Understanding discrimination by the ribosome: stability testing and groove measurement of codon-anticodon pairs.},
  author={Karissa Y. Sanbonmatsu and Simpson Joseph},
  journal={Journal of molecular biology},
  year={2003},
  volume={328 1},
  pages={33-47}
}
The ribosome must discriminate between correct and incorrect tRNAs with sufficient speed and accuracy to sustain an adequate rate of cell growth. Here, we report the results of explicit solvent molecular dynamics simulations, which address the mechanism of discrimination by the ribosome. The universally conserved 16S rRNA base A1493 and the kink in mRNA between A and P sites amplify differences in stability between cognate and near-cognate codon-anticodon pairs. Destabilization by the mRNA kink… CONTINUE READING