Uncovering regulatory pathways that affect hematopoietic stem cell function using 'genetical genomics'

@article{Bystrykh2005UncoveringRP,
  title={Uncovering regulatory pathways that affect hematopoietic stem cell function using 'genetical genomics'},
  author={Leonid V. Bystrykh and Ellen Weersing and Bert H. J. Dontje and Susan E. Sutton and Mathew T. Pletcher and Tim Wiltshire and Andrew I. Su and Edo Vellenga and Jintao Wang and Kenneth F. Manly and Lu Lu and Elissa J. Chesler and Rudi Alberts and Ritsert C. Jansen and Robert W. Williams and Michael P. Cooke and Gerald de Haan},
  journal={Nature Genetics},
  year={2005},
  volume={37},
  pages={225-232}
}
We combined large-scale mRNA expression analysis and gene mapping to identify genes and loci that control hematopoietic stem cell (HSC) function. We measured mRNA expression levels in purified HSCs isolated from a panel of densely genotyped recombinant inbred mouse strains. We mapped quantitative trait loci (QTLs) associated with variation in expression of thousands of transcripts. By comparing the physical transcript position with the location of the controlling QTL, we identified polymorphic… 
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Chapter combInIng transcrIPtIonal ProfIlIng and genetIc lInkage analysIs to uncover gene networks oPeratIng In hematoPoIetIc stem cells and theIr Progeny
TLDR
It is proposed that the combined use of transcriptional profiling and genetic linkage analysis can be a valuable tool to assist in the identification of genes and gene networks that specify “stemness” and cell fate decisions.
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