Uncoupling the roles of synaptotagmin I as a dual Ca2+ sensor during endo- and exocytosis of synaptic vesicles

@article{Yao2012UncouplingTR,
  title={Uncoupling the roles of synaptotagmin I as a dual Ca2+ sensor during endo- and exocytosis of synaptic vesicles},
  author={Jun Yao and Sung E. Kwon and Jon D. Gaffaney and F. Mark Dunning and Edwin R. Chapman},
  journal={Nature neuroscience},
  year={2012},
  volume={15},
  pages={243 - 249}
}
Synaptotagmin I (syt1) is required for normal rates of synaptic vesicle endo- and exocytosis. However, whether the kinetic defects observed during endocytosis in Syt1 knockout neurons are secondary to defective exocytosis or whether syt1 directly regulates the rate of vesicle retrieval remains unknown. To address this question, we sought to dissociate these two activities. We uncoupled the function of syt1 in exo- and endocytosis in mouse neurons either by re-targeting the protein or via… Expand
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