Uncoupling of transpositional immunity from gamma delta transposition by a mutation at the end of gamma delta
@article{Wiater1990UncouplingOT, title={Uncoupling of transpositional immunity from gamma delta transposition by a mutation at the end of gamma delta}, author={Lawrence A. Wiater and Nigel D. F. Grindley}, journal={Journal of Bacteriology}, year={1990}, volume={172}, pages={4959 - 4963} }
The transposon gamma delta, in common with other members of the Tn3 family, confers transpositional immunity, a phenomenon by which plasmids containing a single transposon end show reduced activity as targets for further insertion by the same element. We found that a copy of a mutant delta end, in which the two terminal base pairs (5' GG) were substituted with cytosines, conferred the same degree of immunity as the unaltered delta end. However, a transposon analog with the mutant delta end as…
11 Citations
Integration host factor increases the transpositional immunity conferred by gamma delta ends
- BiologyJournal of bacteriology
- 1990
IHF stimulated the immunity of those plasmids that contain an end of gamma delta, provided the end included the terminal IHF-binding site, and the degree of stimulation of immunity was similar to the stimulation of binding of transposase by IHF.
The Tn3-family of Replicative Transposons.
- BiologyMicrobiology spectrum
- 2015
The dynamic aspects inherent to the diversity and mosaic structure of Tn3-family transposons and their derivatives are discussed, and the current view of the DNA site-specific recombination mechanisms responsible for converting replicative transposition intermediates into final products is summarized.
Surveying a supercoil domain by using the gamma delta resolution system in Salmonella typhimurium
- BiologyJournal of bacteriology
- 1996
It is found that res sites can be plectonemically interwound over long distances ( > 100 kb) and that barriers to supercoil diffusion are placed stochastically within the 43- to 45-min region of the chromosome.
Functional analysis of the two domains in the terminal inverted repeat sequence required for transposition of Tn3.
- Chemistry, BiologyGene
- 1991
Characterization of a Class II Defective Transposon Carrying Two Haloacetate Dehalogenase Genes from Delftia acidovorans Plasmid pUO1
- BiologyApplied and Environmental Microbiology
- 2002
It is demonstrated that Tn Had2 is a defective Tn21-related transposon carrying another class I catabolic transposons, and was defective in transposition because of its lacking the transposase and resolvase genes.
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