Uncoupling of transpositional immunity from gamma delta transposition by a mutation at the end of gamma delta

@article{Wiater1990UncouplingOT,
  title={Uncoupling of transpositional immunity from gamma delta transposition by a mutation at the end of gamma delta},
  author={Lawrence A. Wiater and Nigel D. F. Grindley},
  journal={Journal of Bacteriology},
  year={1990},
  volume={172},
  pages={4959 - 4963}
}
The transposon gamma delta, in common with other members of the Tn3 family, confers transpositional immunity, a phenomenon by which plasmids containing a single transposon end show reduced activity as targets for further insertion by the same element. We found that a copy of a mutant delta end, in which the two terminal base pairs (5' GG) were substituted with cytosines, conferred the same degree of immunity as the unaltered delta end. However, a transposon analog with the mutant delta end as… 
Integration host factor increases the transpositional immunity conferred by gamma delta ends
TLDR
IHF stimulated the immunity of those plasmids that contain an end of gamma delta, provided the end included the terminal IHF-binding site, and the degree of stimulation of immunity was similar to the stimulation of binding of transposase by IHF.
The Tn3-family of Replicative Transposons.
TLDR
The dynamic aspects inherent to the diversity and mosaic structure of Tn3-family transposons and their derivatives are discussed, and the current view of the DNA site-specific recombination mechanisms responsible for converting replicative transposition intermediates into final products is summarized.
Surveying a supercoil domain by using the gamma delta resolution system in Salmonella typhimurium
TLDR
It is found that res sites can be plectonemically interwound over long distances ( > 100 kb) and that barriers to supercoil diffusion are placed stochastically within the 43- to 45-min region of the chromosome.
Characterization of a Class II Defective Transposon Carrying Two Haloacetate Dehalogenase Genes from Delftia acidovorans Plasmid pUO1
TLDR
It is demonstrated that Tn Had2 is a defective Tn21-related transposon carrying another class I catabolic transposons, and was defective in transposition because of its lacking the transposase and resolvase genes.
Insertion sequences.
Stress-induced transposition of Tn4652 in Pseudomonas putida
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References

SHOWING 1-10 OF 28 REFERENCES
Transposition of the Escherichia coli insertion element gamma generates a five-base-pair repeat.
TLDR
It is found that the 35-base-pair inverted repeat that comprises the termini of gamma delta is strikingly similar in sequence to the ends of both the ampicillin-resistance transposon Tn3 and a 200-nucleotide-long sequence on the plasmid pSC101 which has been shown to mediate recombination with phage f1 replicative form.
Nucleotide sequences required for Tn3 transposition immunity
TLDR
The cis-acting site on Tn3 responsible for immunity was mapped by deletions from each side to be within the terminal 38-base-pair sequence that is inversely repeated at the ends of Tn2.
Identification of a transposon Tn3 sequence required for transposition immunity.
TLDR
The ability of Tn3 to form cointegrates between two plasmids to develop a quantitative assay to detect transposition immunity and it is determined that tnpR is not required for immunity, and only the terminal 38 base pairs of TN3 need be present to confer immunity.
Genetic analysis of the interaction of the insertion sequence IS903 transposase with its terminal inverted repeats.
TLDR
The insertion sequence IS903 has perfect, 18-base-pair terminal repeats that are the presumed binding sites of its transposase and it is inferred that the inverted repeat contains two functional domains--one for initial complex formation with transpos enzyme and the other for effective completion of transpositional recombination.
Bacteriophage Mu sites required for transposition immunity.
TLDR
Transposition immunity was seen both during full phage lytic growth, with all the bacteriophage Mu genes, and during normal cellular Growth, with a mini-Mu element containing only the Mu c and ner regulatory and A and B transposition genes.
Mutational analysis of IS10′s outside end.
TLDR
The phenotypes of mutations in this region suggest that IHF is the major host factor for outside‐end transposition activity in vivo and that base pairs throughout this region are important for the IHF interaction.
The λδ sequence of F is an insertion sequence
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