Uncoupler resistance in E. coli Tuv and Cuv is due to the exclusion of uncoupler by the outer membrane.

@article{Haworth1990UncouplerRI,
  title={Uncoupler resistance in E. coli Tuv and Cuv is due to the exclusion of uncoupler by the outer membrane.},
  author={Robert S. Haworth and Peter Ruhdal Jensen and Ole Michelsen and John A. Wyatt and Clive J. Brealey and R. Brian Beechey},
  journal={Biochimica et biophysica acta},
  year={1990},
  volume={1019 1},
  pages={
          67-72
        }
}
The uncoupler resistant bacterial strains E. coli Tuv and Cuv share the high deoxycholate sensitivity of the parent strain, Doc S. However, both Tuv and Cuv show greater resistance than Doc S to other detergents. Measurement of the periplasmic volume indicates that the outer membrane of Doc S is freely permeable to both TPP+ and hydroxymethylinulin. Tuv and Cuv are able to exclude these compounds. EDTA treatment was necessary prior to measuring membrane potential in Tuv and Cuv. Under… 
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It is suggested that the active resistance of strain TUV results from the exclusion of uncoupler by the interaction of inner and outer membrane components in a manner modulated by the degree of cellular energization.
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TLDR
Two mutant strains of E. coli resistant to the uncoupling agents 4,5,6,7- tetrachloro-2-trifluoromethyl benzimidazole and carbonyl cyanide m-cblorophenylhydrazone were isolated and it is concluded that uncoupler resistance in these strains has arisen as a consequence of mutations which directly affect a specific site of unc Coupler action within the cytoplasmic membrane.
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The experiments here support the hypothesis developed from work with Bacillus subtilis that changes in the fatty acid composition of the membrane phospholipids affect energy coupling, and make it clear that simple increases or decreases in the hydrolytic activity of ATPase in the uncoupler-resistant mutants of bacilli are not correlated with resistance in some direct way.
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