Uncoating of Clathrin-Coated Vesicles in Presynaptic Terminals Roles for Hsc70 and Auxilin

@article{Morgan2001UncoatingOC,
  title={Uncoating of Clathrin-Coated Vesicles in Presynaptic Terminals Roles for Hsc70 and Auxilin},
  author={Jennifer R. Morgan and K. Hari Prasad and Suping Jin and George J. Augustine and Eileen M. Lafer},
  journal={Neuron},
  year={2001},
  volume={32},
  pages={289-300}
}
We have examined the roles of Hsc70 and auxilin in the uncoating of clathrin-coated vesicles (CCVs) during neuronal endocytosis. We identified two peptides that inhibit the ability of Hsc70 and auxilin to uncoat CCVs in vitro. When injected into nerve terminals, these peptides inhibited both synaptic transmission and CCV uncoating. Mutation of a conserved HPD motif within the J domain of auxilin prevented binding to Hsc70 in vitro and injecting this mutant protein inhibited CCV uncoating in… CONTINUE READING