Unambiguous stereochemical course of rabbit liver fructose bisphosphatase hydrolysis.

Abstract

The stereochemical course of rabbit liver fructose bisphosphatase (EC 3.1.3.11) was determined by hydrolyzing the substrate analogue (Sp)-[1-18O]fructose 1-phosphorothioate 6-phosphate in H(2)17O, incorporating the chiral, inorganic phosphorothioate product into adenosine 5'-O-(2-thiotriphosphate) (ATP beta S), and analyzing the isotopic distribution of 18O… (More)

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@article{Domanico1985UnambiguousSC, title={Unambiguous stereochemical course of rabbit liver fructose bisphosphatase hydrolysis.}, author={Paul L. Domanico and Jubrail Rahil and Silvija Benkovic}, journal={Biochemistry}, year={1985}, volume={24 7}, pages={1623-8} }