Uml2 is a novel CalB-type lipase of Ustilago maydis with phospholipase A activity

@article{Buerth2013Uml2IA,
  title={Uml2 is a novel CalB-type lipase of Ustilago maydis with phospholipase A activity},
  author={Christoph Buerth and Filip Kova{\vc}i{\'c} and Janpeter Stock and Marius Terfr{\"u}chte and Susanne Wilhelm and K. -E. J{\"a}ger and Michael Feldbr{\"u}gge and Kerstin Schipper and J. F. Ernst and Denis Tielker},
  journal={Applied Microbiology and Biotechnology},
  year={2013},
  volume={98},
  pages={4963-4973}
}
CalB of Pseudozyma aphidis (formerly named Candida antarctica) is one of the most widely applied enzymes in industrial biocatalysis. Here, we describe a protein with 66 % sequence identity to CalB, designated Ustilago maydis lipase 2 (Uml2), which was identified as the product of gene um01422 of the corn smut fungus U. maydis. Sequence analysis of Uml2 revealed the presence of a typical lipase catalytic triad, Ser-His-Asp with Ser125 located in a Thr-Xaa-Ser-Xaa-Gly pentapeptide. Deletion of… CONTINUE READING
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