Ultraviolet D I F F E R E N C E Spectra of Pepsin*,$ the Journal of General Physiology

A shift of pH of pepsin solutions from 4.6 to 1.0 gives rise to spectral displacemerits in the ultraviolet. If represented as difference spectra three peaks with maxima at 2770, 2850, and 2930 ,~mgstr~ms are present which can be attributed to the tyrosine and tryptophan residues in the protein. On mild autolysis of pepsin at pH 2.0 the absorbancy in the… CONTINUE READING