Ultrathin cryosections in the plane of cell monolayers: evaluation of their potential for antibody localization studies of the cytoskeleton.

In spite of the fact that the preembedding method is satisfactory for the ultrastructural localization of cytoskeletal proteins, there is a need for a localization method that retains the cells' ground substance, delicate filament arrangements, and membrane-filament interactions and provides a good delineation of ultrastructural detail. Ultracryomicrotomy… (More)