Ultrastructure ofBlastocystis hominis cysts

@article{Zaman2004UltrastructureOH,
  title={Ultrastructure ofBlastocystis hominis cysts},
  author={Viqar Zaman and Josephine Howe and M. Ng},
  journal={Parasitology Research},
  year={2004},
  volume={81},
  pages={465-469}
}
Blastocystis hominis cysts were concentrated from human faeces by repeated washing in distilled water and centrifugation on Ficoll-Paque. This procedure gave a concentrated suspension of cysts without yielding any non-cystic forms. The cysts were examined by both transmission (TEM) and scanning electron microscopy (SEM). Cysts were surrounded by a fibrillar layer that appeared porous in SEM. Many naked cysts without the fibrillar layer were also visible in SEM. They were variable in shape but… Expand

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References

SHOWING 1-7 OF 7 REFERENCES
Ultrastructure of Blastocystis hominis in human stool samples.
TLDR
Results suggest that the vacuolated form of Blastocystis hominis may be an artefact of culture conditions, and that the form of B. hominis present in the gastrointestinal tract is avacuolar. Expand
A cyst-like stage of Blastocystis hominis.
TLDR
A cyst-like form of Blastocystis hominis is described in stools and in culture that can withstand adverse environmental conditions and is infective to a new host remain to be determined. Expand
Blastocystis hominis--past and future.
  • C. Zierdt
  • Medicine, Biology
  • Clinical Microbiology Reviews
  • 1991
TLDR
The pathology of B. hominis infections has been studied in gnotobiotic guinea pigs in which inflammation of the intestinal mucosa and invasion of the superficial layers were seen and limited studies of human pathology are available. Expand
Chromosomes of Blastocystis hominis.
Three stocks of Blastocystis hominis were adapted to monophasic culture in minimal essential medium (MEM) and the chromosomes of these stocks separated by field inversion gel electrophoresis (FIGE).Expand
Isolation ofBlastocystis from the cockroach (Dictyoptera: Blattidae)
TLDR
Investigation of the cockroach as a possible vector would be of interest, as the mode of infection in humans is not known, and eight isolates of Blastocystis were obtained, indicating a high prevalence rate. Expand
Blastocystis hominis, Brumpt 1912: Are there different stages or species?
The experimental infection of rats with Entamoeba histolytica; with a method for evaluating the anti-amoebic properties of new compounds.
  • W. Jones
  • Biology, Medicine
  • Annals of tropical medicine and parasitology
  • 1946