Ultrastructure of in vitro oocyte maturation in buffalo (Bubalus bubalis).

  title={Ultrastructure of in vitro oocyte maturation in buffalo (Bubalus bubalis).},
  author={Rafael Gianella Mondadori and Tiago Rollemberg Santin and Andrei Antonioni Guedes Fidelis and Khesller Patr{\'i}cia Ol{\'a}zia Name and Juliana Souza da Silva and Rodolfo Rumpf and S{\^o}nia Nair B{\'a}o},
  volume={18 4},
The objective of the present study was to describe ultrastructural changes in the nucleus and cytoplasmic organelles during in vitro maturation (IVM) of buffalo cumulus-oocyte complexes (COCs). The structures were collected by ovum pick-up (OPU). Some COCs, removed from maturation medium at 0, 6, 12, 18 and 24 h, were processed for transmission electron microscopy. The average number of COCs collected by OPU/animal/session was 6.4, and 44% of them were viable. Immature oocytes had a… CONTINUE READING