Ultrastructure of human Kupffer cells maintained in culture.

  title={Ultrastructure of human Kupffer cells maintained in culture.},
  author={V Neaud and Liliane Dubuisson and Charles Balabaud and Paulette Bioulac-Sage},
  journal={Journal of submicroscopic cytology and pathology},
  volume={27 2},
Sinusoidal cells were isolated by collagenase perfusion and metrizamide gradient centrifugation, from liver resected for partial hepatectomy performed under warm ischemic conditions. Kupffer cells were then separated from this population by centrifugal elutriation. Isolated Kupffer cells showed good viability, with the typical features of Kupffer cells and were engaged in the endocytosis of foreign particles. They showed numerous morphological criteria of activation. However, cultured Kupffer… CONTINUE READING

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