Ultrastructure and histochemical analysis of extracellular matrix surface network in kiwifruit endosperm-derived callus culture

@article{PopielarskaKonieczna2008UltrastructureAH,
  title={Ultrastructure and histochemical analysis of extracellular matrix surface network in kiwifruit endosperm-derived callus culture},
  author={Marzena Popielarska-Konieczna and Małgorzata Kozieradzka-Kiszkurno and Joanna Świerczyńska and Grzegorz G{\'o}ralski and Halina Ślesak and Jerzy Bohdanowicz},
  journal={Plant Cell Reports},
  year={2008},
  volume={27},
  pages={1137-1145}
}
The study used Actinidia deliciosa endosperm-derived callus to investigate aspects of the morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM showed ECM as a membranous layer or reticulated fibrillar and granular structure linking the peripheral cells of callus domains. TEM confirmed that ECM is a distinct heterogeneous layer, up to 4 μm thick and consisting of amorphous dark-staining material, osmiophilic… CONTINUE READING