The ultrastructure, development, and morphogenesis of the body cuticle of adult and juvenile Oesophagostomum dentatum (Strongylida, Nematoda) were investigated by light microscopy, scanning electron microscopy, and transmission electron microscopy. The cuticle of the first 3 juvenile stages consists of a trilaminate epicuticle, an amorphous layer, and a radially striated layer. In the last juvenile stage and the adult worm, the radially striated layer is replaced by a fibrous layer with 3 sublayers of giant fibers and a basal amorphous layer. The new forming cuticle of the 3rd juvenile stage exhibits the epicuticle and amorphous material and, at a later time, the epicuticle and the radially striated layer; finally, amorphous material appears between these layers. Our finding of a structural change in the cuticular morphology between the 3rd and 4th juvenile stage harmonizes with earlier reports about the Strongylida. Such a change occurs at different ontogenetic stages or seems to be missing in other nematodes. Morphogenetic events such as the formation of the radial striation layer from amorphous precursor material agrees with previous observations on strongylids.