Ultrastructural study ofCryptococcus neoformans by quick-freezing and deep-etching method

  title={Ultrastructural study ofCryptococcus neoformans by quick-freezing and deep-etching method},
  author={Dr Nobuki Sakaguchi and Takeshi Baba and Masao Fukuzawa and Shinichi Ohno},
The three-dimensional ultrastructure ofCryptococcus neoformans was studied by quick-freezing and deep-etching (QF-DE) method.C. neoformans, strain CDC551, was cultured on agar. The viable yeast cells (107 cells) were inoculated into each mouse from the tail vein. Three weeks after the inoculation, the brains of the mice were perfused with fixatives, quickly frozen, freeze-fractured, deeply etched and rotary shadowed with platinum and carbon. In addition, the viable cells ofC. neoformans on agar… CONTINUE READING


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