Ultrastructural observations on gamete interactions using micromanipulated mouse oocytes.

@article{Cummins1989UltrastructuralOO,
  title={Ultrastructural observations on gamete interactions using micromanipulated mouse oocytes.},
  author={James M. Cummins and W. Rohini Edirisinghe and Yoshihiro Odawara and Roxana Wales and John L Yovich},
  journal={Gamete research},
  year={1989},
  volume={24 4},
  pages={461-9}
}
Cumulus-free mouse oocytes were subjected to zona opening by cracking with microhooks (ZC) or acid drilling (ZD) and fixed 30-90 min after insemination (10(5) pre-capacitated motile sperms/ml). Ultrastructural observations were made on serially thin-sectioned oocytes: 15 ZC and 12 ZD. The zona lesion in ZC oocytes was a clean cut, whereas in ZD oocytes it formed a patchy area of partial zona loss, with reduced microvillar height on the underlying oocyte surface. Spermatozoa were observed within… CONTINUE READING