Ultrastructural localization of steroid sulphatase in cultured human fibroblasts by immunocytochemistry: A comparative study with lysosomal enzymes and the mannose 6-phosphate receptor

@article{Willemsen1988UltrastructuralLO,
  title={Ultrastructural localization of steroid sulphatase in cultured human fibroblasts by immunocytochemistry: A comparative study with lysosomal enzymes and the mannose 6-phosphate receptor},
  author={Rob Willemsen and Marian A. Kroos and Andr{\'e} T. Hoogeveen and Jacques J. M. van Dongen and Giuliano Francesco Parenti and Chris M. van der Loos and Arnold J. J. Reuser},
  journal={The Histochemical Journal},
  year={1988},
  volume={20},
  pages={41-51}
}
Immunocytochemistry was used to study the subcellular localization of steroid sulphatase in cultured human fibroblasts. Ultra-thin cryosections were incubated with antibodies raised against steroid sulphatase purified from human placenta and immune complexes were visualized with gold probes as electron dense markers. Steroid sulphatase was found in rough endoplasmic reticulum, Golgi cisternae and in the trans-Golgi reticulum, where it co-distributes with lysosomal enzymes and the mannose 6… CONTINUE READING