Ultrastructural localization of daunomycin in multidrug-resistant cultured cells with modulation of the multidrug transporter.

@article{Rutherford1993UltrastructuralLO,
  title={Ultrastructural localization of daunomycin in multidrug-resistant cultured cells with modulation of the multidrug transporter.},
  author={A V Rutherford and Mark C. Willingham},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1993},
  volume={41 10},
  pages={1573-7}
}
We localized the chemotherapeutic drug daunomycin inside cultured cells by taking advantage of its inherent fluorescence. Multidrug-resistant cultured cells, in which the accumulation of daunomycin can be reversibly controlled with verapamil to block the multidrug transporter, were incubated in daunomycin and verapamil and the accumulated daunomycin was visualized with epifluorescence optics. After fixation under a variety of different conditions to make cells permeable to diaminobenzidine (DAB… CONTINUE READING