Ultrastructural localization of calcium in the CNS of vertebrates

  title={Ultrastructural localization of calcium in the CNS of vertebrates},
  author={Wolfgang Probst},
The ultrastructural localization of calcium in synaptic areas of the CNS of fish was investigated. Prefixation with phosphate-buffered glutaraldehyde followed by post-fixation with osmium/potassium-bichromate was used to precipitate and visualize endogenous calcium without the addition of external calcium. The presence of calcium in the electron-dense precipitates produced using this method was demonstrated by electron spectroscopic imaging using a Zeiss EM-902 transmission electron microscope… CONTINUE READING


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