Ultrastructural localization and distribution of free cholesterol (3-beta-hydroxysterols) in tissues.

  title={Ultrastructural localization and distribution of free cholesterol (3-beta-hydroxysterols) in tissues.},
  author={Joseph R. Williamson},
  journal={Journal of ultrastructure research},
  volume={27 2},
A method is described for the ultrastructural demonstration of free cholesterol in glutaraldehyde-fixed tissues by the use of digitonin. Characteristic reaction products permit localization of free cholesterol in extracellular as well as intracellular compartments. In numerous cell types, digitonin-induced alterations indicated the presence of cholesterol focally in plasma membranes and in membranes of nuclei, rough endoplasmic reticulum, lysosomes, secretory granules, and cytoplasmic vacuoles… CONTINUE READING

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