Ultrastructural localization and distribution of free cholesterol (3-beta-hydroxysterols) in tissues.

@article{Williamson1969UltrastructuralLA,
  title={Ultrastructural localization and distribution of free cholesterol (3-beta-hydroxysterols) in tissues.},
  author={Joseph R. Williamson},
  journal={Journal of ultrastructure research},
  year={1969},
  volume={27 2},
  pages={
          118-33
        }
}
A method is described for the ultrastructural demonstration of free cholesterol in glutaraldehyde-fixed tissues by the use of digitonin. Characteristic reaction products permit localization of free cholesterol in extracellular as well as intracellular compartments. In numerous cell types, digitonin-induced alterations indicated the presence of cholesterol focally in plasma membranes and in membranes of nuclei, rough endoplasmic reticulum, lysosomes, secretory granules, and cytoplasmic vacuoles… CONTINUE READING

Topics from this paper.