Ultrastructural localisation of calcium deposits in pig oocytes maturing in vitro: effects of verapamil.

@article{Rozinek2003UltrastructuralLO,
  title={Ultrastructural localisation of calcium deposits in pig oocytes maturing in vitro: effects of verapamil.},
  author={Jir{\'i} Rozinek and Zdena Vanourkov{\'a} and Mark{\'e}ta Sedm{\'i}kov{\'a} and Vilma L{\'a}nsk{\'a} and Jaroslav Petr and Radko Rajmon and Franti{\vs}ek J{\'i}lek},
  journal={Zygote},
  year={2003},
  volume={11 3},
  pages={253-60}
}
The culture of pig oocytes in the presence of the calcium channel blocker verapamil (0.02 mM) resulted in the blocking of meiosis at the metaphase I stage, and only a small fraction (about 28%) of the oocytes were able to continue their maturation to the stage of metaphase II. Hence, meiotic maturation in pig oocytes is a calcium-dependent process. After isolation of the pig oocytes from their follicles, the intracellular calcium deposits in the oocyte and granulosa cells, detectable using the… CONTINUE READING