Ultrastructural immunogold localization of tumor necrosis factor-alpha to the cytoplasmic granules of rat peritoneal mast cells with rapid microwave fixation.

@article{Beil1994UltrastructuralIL,
  title={Ultrastructural immunogold localization of tumor necrosis factor-alpha to the cytoplasmic granules of rat peritoneal mast cells with rapid microwave fixation.},
  author={Waltraud J. Beil and Gary R Login and Stephen J. Galli and Ann M. Dvorak},
  journal={The Journal of allergy and clinical immunology},
  year={1994},
  volume={94 3 Pt 1},
  pages={531-6}
}
Tumor necrosis factor-alpha (TNF-alpha) is a multifunctional, proinflammatory cytokine, which can be produced by mast cells and several other cell types. We used a newly developed microwave energy-assisted aldehyde fixation method to prepare purified rat peritoneal mast cells for the postembedding immunogold ultrastructural localization of TNF-alpha. These fixation methods were superior to chemical fixation alone in preserving both the ultrastructural morphology and immunoreactive TNF-alpha in… CONTINUE READING

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