Ultrastructural evidence for direct projections from the pontine micturition center to glycine‐immunoreactive neurons in the sacral dorsal gray commissure in the cat

  title={Ultrastructural evidence for direct projections from the pontine micturition center to glycine‐immunoreactive neurons in the sacral dorsal gray commissure in the cat},
  author={J A Sie and Bertil F. M. Blok and Hendrik A. de Weerd and Gert Holstege},
  journal={Journal of Comparative Neurology},
During micturition, according to the concept of Blok, Holstege, and colleagues ([1997] Neurosci. Lett. 233:109–112), the pontine micturition center (PMC) elicits bladder contraction by way of direct excitation of the parasympathetic bladder motoneurons. At the same time, the PMC elicits relaxation of the external urethral sphincter (EUS) by excitation of γ‐aminobutyric acid (GABA)‐ergic interneurons in the sacral dorsal gray commissure (DGC), which, in turn, inhibit EUS motoneurons. The… 

Afferent projections to the pontine micturition center in the cat

All other parts of the brain that influence micturition have no direct connection with the PMC, as indicated by results in 67 cats.

Morphologic relationship between the pontine micturition center and the sympathetic center in the spinal cord of the rat

It is concluded that PMC may control the preganglionic neurons of sympathetic nerves through the interneurons located dorsal to PPNs.

Micturition and the soul

  • G. Holstege
  • Biology
    The Journal of comparative neurology
  • 2005
It seems that the many structures in the brain that are known to influence micturition use the PAG as relay to the PMC, which has to be kept in mind in the fight against overactive bladder (OAB) and urge‐incontinence.

Sacral dorsal horn neurone activity during micturition in the cat

The excitability of two groups of neurones located in different parts of the sacral spinal cord were examined during micturition in decerebrate adult cats to hypothesized that these sacral neurones are interposed in polysynaptic excitatory pathways from sacral perineal afferents to sphincter motoneurones and that they are subject to direct postsynaptic inhibition during mitzurition.

Neural control of the lower urinary and gastrointestinal tracts: Supraspinal CNS mechanisms

Normal urinary function is contingent upon a complex hierarchy of CNS regulation, and alterations in cognitive modulation, descending modulation, and hypervigilance are important in functional (symptom‐based) clinical disorders.

Correlation between electrophysiology and morphology of three groups of neuron in the dorsal commissural nucleus of lumbosacral spinal cord of mature rats studied in vitro

Electrophysiological and morphological properties of neurons in the DCN of L6‐S1 were examined using whole‐cell recordings with biocytin‐filled electrodes in transverse slices of mature rat spinal cord, and phasic neurons had the most elaborate dendritic branching and the largest numbers of axon collaterals.

Rectal Distention Inhibits the Spinal Micturition Reflex via Glycinergic or GABAergic Mechanisms in Rats with Spinal Cord Injury

There is an inhibitory rectovesical reflex in the lumbosacral cord of rats with spinal cord injury, which modulates the spinal micturition reflex via glycinergic or GABAergic mechanisms.

Barrington's nucleus: Neuroanatomic landscape of the mouse “pontine micturition center”

Barrington's nucleus (Bar) is thought to contain neurons that trigger voiding and thereby function as the “pontine micturition center.” Lacking detailed information on this region in mice, we



Evidence for a strychnine-sensitive mechanism and glycine receptors involved in the control of urethral sphincter activity during micturition in the cat

It was concluded that a strychnine-sensitive mechanism contributes to the suppression of sphincter activity normally observed during voiding, and it appears that the suppressed EUS motoneurons during micturition may be partly due to a direct glycinergic inhibition of the EUS Motoneuron populations.

Glycine‐immunoreactive synaptic terminals in the nucleus tractus solitarii of the cat: Ultrastructure and relationship to GABA‐immunoreactive terminals

This study reports the first analysis of the ultrastructure of glycinergic nerve terminals in the cat dorsal vagal complex, and the pattern of coexistence of glycine and GABA observed provides an anatomical explanation for the previously reported inhibitory effects of glucose and GABA on neurones with cardiovascular and respiratory functions in the nucleus tractus solitarii.

Anatomical and physiological observations on suprapinal control of bladder and urethral sphincter muscles in the cat

In 15 cats injections of 3H‐leucine made in the pontine tegmentum resulted in specific projections to the sacral intermediomedial and intermediolateral cell groups, which contains preganglionic parasympathetic neurons that form the motor supply of the detrusor muscle of the bladder.

Immunohistochemical evidence for coexistence of glycine and GABA in nerve terminals on cat spinal motoneurones: an ultrastructural study.

It is shown that glycine- and GABA-immunoreactive nerve terminals occur in contact with cell bodies of alpha motoneurone size, as well as in the surrounding neuropil in the motor nuclei of the cat spinal cord.

Distribution pattern of inhibitory and excitatory synapses in the dendritic tree of single masseter α‐motoneurons in the cat

There is possible evidence that the spatial distribution patterns of inhibitory and excitatory synapses are different in the dendritic tree of jaw‐closing α‐motoneurons in the brainstem and spinal cord.

Neurons in the rat brain and spinal cord labeled after pseudorabies virus injected into the external urethral sphincter

Male Sprague‐Dawley rats, with their pelvic and hypogastric nerves transected, were infected with pseudorabies virus injected into the external urethral sphincter and the distribution of these PRV‐labeled brain neurons strongly resembled that obtained after the injection of PRV into the urinary bladder.

Bladder and urethral pressures evoked by microstimulation of the sacral spinal cord in cats