Ultrastructural changes associated with cryopreservation of potato (Solanum tuberosum l.) shoot tips.

@article{Kaczmarczyk2008UltrastructuralCA,
  title={Ultrastructural changes associated with cryopreservation of potato (Solanum tuberosum l.) shoot tips.},
  author={Anja Kaczmarczyk and Twan Rutten and Michael Karl Melzer and E. R. Joachim Keller},
  journal={Cryo letters},
  year={2008},
  volume={29 2},
  pages={145-56}
}
The ultrastructure of cells within shoot tips of S. tuberosum 'Désirée' was studied after different steps of the DMSO droplet cryopreservation method. After 2 h of DMSO treatment, cells contained numerous small vesicles, while at the same time mitochondria and chloroplasts had increased in size and vacuoles had assumed an irregular shape. After rapid cooling in liquid nitrogen, subsequent rewarming, and 1 h incubation there were no apparent changes in the ultrastructural organization of the… CONTINUE READING
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