Ultrastructural, immunohistological and biochemical characterization of cultured mouse corneal epithelial cells.

@article{Hazlett1996UltrastructuralIA,
  title={Ultrastructural, immunohistological and biochemical characterization of cultured mouse corneal epithelial cells.},
  author={Linda D Hazlett and S A Masinick and B Mezger and Ronald E. Barrett and Michelle A. Kurpakus and Marilyn Garrett},
  journal={Ophthalmic research},
  year={1996},
  volume={28 1},
  pages={50-6}
}
We developed a method to culture mouse corneal epithelium. Cultured cells tested by 1-D-SDS-PAGE exhibited protein mobility patterns similar to freshly isolated epithelia. Western blots with antibodies broadly recognizing cytokeratins showed a similar pattern for both fresh and cultured cells, but only the fresh sample stained with J7, specific for a 55-kD 'corneal' cytokeratin. Cultured cells examined at confluency by transmission electron microscopy exhibited desmosomal contacts typical of… CONTINUE READING

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