Ultrasound and microbubble-targeted delivery of macromolecules is regulated by induction of endocytosis and pore formation.

@article{Meijering2009UltrasoundAM,
  title={Ultrasound and microbubble-targeted delivery of macromolecules is regulated by induction of endocytosis and pore formation.},
  author={Bernadet D. M. Meijering and Lynda J. M. Juffermans and Annemieke van Wamel and Rob H. Henning and Inge S. Zuhorn and Marcia Emmer and Amanda M. G. Versteilen and Walter Paulus and Wiek H van Gilst and Klazina Kooiman and Nico de Jong and Ren{\'e} John Philip Musters and Leo E Deelman and Otto Kamp},
  journal={Circulation research},
  year={2009},
  volume={104 5},
  pages={679-87}
}
Contrast microbubbles in combination with ultrasound (US) are promising vehicles for local drug and gene delivery. However, the exact mechanisms behind intracellular delivery of therapeutic compounds remain to be resolved. We hypothesized that endocytosis and pore formation are involved during US and microbubble targeted delivery (UMTD) of therapeutic compounds. Therefore, primary endothelial cells were subjected to UMTD of fluorescent dextrans (4.4 to 500 kDa) using 1 MHz pulsed US with 0.22… CONTINUE READING