Ultrasonic analysis of kinetic mechanism of hydrolysis of cellobiose by β-glucosidase.


High-resolution ultrasonic spectroscopy (HR-US) was applied for real-time analysis of enzymatic hydrolysis of cellobiose by a β-glucosidase from Aspergillus niger (Novozyme 188) at 50°C and pH 4.9. This technique is noninvasive, it does not require optical transparency and is suitable to continuously monitor the time dependence of the reaction progress in a… (More)
DOI: 10.1016/j.ab.2011.03.003


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