Ultrasensitive optical DNA biosensor based on surface immobilization of molecular beacon by a bridge structure.

@article{Li2001UltrasensitiveOD,
  title={Ultrasensitive optical DNA biosensor based on surface immobilization of molecular beacon by a bridge structure.},
  author={Jianzhu Li and Weihong Tan and K Wang and Dan Xiao and Xiaoming Yang and Xiaoxiao He and Zichao Tang},
  journal={Analytical sciences : the international journal of the Japan Society for Analytical Chemistry},
  year={2001},
  volume={17 10},
  pages={1149-53}
}
A novel biotinylated molecular beacon (MB) probe was developed to prepare a DNA biosensor using a bridge structure. MB was biotinylated at the quencher side of the stem and linked on a biotinylated glass cover slip through streptavidin, which acted as a bridge between MB and glass matrix. An efficient fluorescence microscope system was constructed to detect the fluorescence change caused by the conformation change of MB in the presence of complementary DNA target. The proposed biosensor was… CONTINUE READING

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