Ultrasensitive biosensing platform based on the luminescence quenching ability of plasmonic palladium nanoparticles.

Abstract

An ultrasensitive biosensing platform for DNA and protein detection is constructed based on the luminescence quenching ability of plasmonic palladium nanoparticles (PdNPs). By growing the particles into large sizes (ca. 30 nm), the plasmonic light absorption of PdNPs is broadened and extended to the visible range with extinction coefficients as high as 10(9)  L mol(-1)  cm(-1) , enabling complete quenching of fluorescent dyes that emit at diverse ranges and that are tagged to bioprobes. Meanwhile the nonspecific quenching of the dyes (not bound to probes) is negligible, leading to extremely low background signal. Utilizing the affinity of PdNPs towards bioprobes, such as single-stranded (ss) DNA and polypeptide molecules, which is mainly assigned to the coordination interaction, nucleic acid assays with a quantification limit of 3 pM target DNA and protein assay are achieved with a simple mix-and-detect strategy based on the luminescence quenching-and-recovery protocol. This is the first demonstration of biosensing employing plasmonic absorption of nanopalladium, which offers pronounced sensing performances and can be reasonably expected for wide applications.

DOI: 10.1002/chem.201406633

Cite this paper

@article{Li2015UltrasensitiveBP, title={Ultrasensitive biosensing platform based on the luminescence quenching ability of plasmonic palladium nanoparticles.}, author={Hui Li and De-En Sun and Zhihong Liu}, journal={Chemistry}, year={2015}, volume={21 13}, pages={4944-8} }