Ultrasensitive and absolute quantification of the phosphoinositide 3-kinase/Akt signal transduction pathway by mass spectrometry.

@article{Cutillas2006UltrasensitiveAA,
  title={Ultrasensitive and absolute quantification of the phosphoinositide 3-kinase/Akt signal transduction pathway by mass spectrometry.},
  author={Pedro R Cutillas and Asim A. Khwaja and Mariona Graupera and Wayne P. Pearce and Severine I. Gharbi and Mike Waterfield and Bart Vanhaesebroeck},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2006},
  volume={103 24},
  pages={8959-64}
}
The phosphoinositide 3-kinase (PI3K)/Akt pathway controls a vast array of normal physiological processes and is frequently aberrantly activated in cancer, thus identifying PI3K/Akt-signaling components as promising drug targets in oncology. However, implementation of rational cancer therapies for this pathway needs robust and simple tools to stratify patients according to PI3K pathway activation and to validate and measure the impact of targeted inhibition on primary cancer tissues. Herein we… CONTINUE READING