Ultrarapid Nanopore Genome Sequencing in a Critical Care Setting.

@article{Gorzynski2022UltrarapidNG,
  title={Ultrarapid Nanopore Genome Sequencing in a Critical Care Setting.},
  author={John E Gorzynski and Sneha D. Goenka and Kishwar Shafin and Tanner D Jensen and Dianna G. Fisk and Megan E Grove and Elizabeth Spiteri and Trevor Pesout and Jean Monlong and Gunjan Baid and Jonathan A Bernstein and Scott R Ceresnak and Pi-Chuan Chang and Jeffrey W. Christle and Henry Chubb and Karen P. Dalton and Kyla Dunn and Daniel R. Garalde and Joseph Guillory and Joshua W. Knowles and Alexey Kolesnikov and Michael Ma and Tia Moscarello and Maria Nattestad and Marco Perez and Maura R.Z. Ruzhnikov and Mehrzad Samadi and Ankit Setia and Chris Wright and Courtney J. Wusthoff and Katherine Xiong and Tong Zhu and Miten Jain and Fritz J. Sedlazeck and Andrew Carroll and Benedict J. Paten and Euan A. Ashley},
  journal={The New England journal of medicine},
  year={2022}
}
To the Editor: Rapid genetic diagnosis can guide clinical management, improve prognosis, and reduce costs in critically ill patients.1,2 Although most critical care decisions must be made in hours, traditional testing requires weeks and rapid testing requires days. We have found that nanopore genome sequencing can accurately and rapidly provide genetic diagnoses. Our workflow combines streamlined preparation of commercial nanopore sequencing, distributed Cloudbased bioinformatics, and a custom… 

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