Ultrahigh-pressure liquid chromatography of isoflavones and phenolic acids on different stationary phases.

@article{Klejdus2008UltrahighpressureLC,
  title={Ultrahigh-pressure liquid chromatography of isoflavones and phenolic acids on different stationary phases.},
  author={Bořivoj Klejdus and Jan Vacek and Lea Lojkov{\'a} and Lucie Benesova and Vlastimil Kub{\'a}ň},
  journal={Journal of chromatography. A},
  year={2008},
  volume={1195 1-2},
  pages={
          52-9
        }
}
Complete separation of aglycones and glucosides of selected isoflavones (genistin, genistein, daidzin, daidzein, glycitin, glycitein, ononin, sissotrin, formononetin, and biochanin A) was possible in 1.5 min using an ultrahigh-pressure liquid chromatography (U-HPLC) on a different particular chemically modified stationary phases with a particle size under 2 microm. In addition, selected separation conditions for simultaneous determination of isoflavones together with a group of phenolic acids… CONTINUE READING

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Proposed U - HPLC technique was used for separation of isoflavones and phenolic acids in samples of plant materials ( Trifolium pratense , Glycine max , Pisum sativum and Ononis spinosa ) after acid hydrolysis of the samples and modified Soxhlet extraction .
Proposed U - HPLC technique was used for separation of isoflavones and phenolic acids in samples of plant materials ( Trifolium pratense , Glycine max , Pisum sativum and Ononis spinosa ) after acid hydrolysis of the samples and modified Soxhlet extraction .
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
Proposed U - HPLC technique was used for separation of isoflavones and phenolic acids in samples of plant materials ( Trifolium pratense , Glycine max , Pisum sativum and Ononis spinosa ) after acid hydrolysis of the samples and modified Soxhlet extraction .
Proposed U - HPLC technique was used for separation of isoflavones and phenolic acids in samples of plant materials ( Trifolium pratense , Glycine max , Pisum sativum and Ononis spinosa ) after acid hydrolysis of the samples and modified Soxhlet extraction .
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
daidzeinNo subtypedaidzin
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
daidzinNo subtypedaidzein
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
Complete separation of aglycones and glucosides of selected isoflavones ( genistin , genistein , daidzin , daidzein , glycitin , glycitein , ononin , sissotrin , formononetin , and biochanin A ) was possible in 1.5 min using an ultrahigh - pressure liquid chromatography ( U - HPLC ) on a different particular chemically modified stationary phases with a particle size under 2 microm .
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