Ultrafast photochemistry of light-adapted and dark-adapted bacteriorhodopsin: effects of the initial retinal configuration.

@article{Wand2012UltrafastPO,
  title={Ultrafast photochemistry of light-adapted and dark-adapted bacteriorhodopsin: effects of the initial retinal configuration.},
  author={Amir Wand and Noga Friedman and Mordechai Sheves and Sanford Ruhman},
  journal={The journal of physical chemistry. B},
  year={2012},
  volume={116 35},
  pages={10444-52}
}
Femtosecond spectroscopy is used to compare photochemical dynamics in light-adapted and dark-adapted bacteriorhodopsin (BR). The retinal prosthetic group is initially all-trans in the former, while it is nearly a 1:1 mixture with 13-cis in the latter. Comparing photochemistry in both serves to assess how the initial retinal configuration influences internal conversion and photoisomerization dynamics. Contrary to an earlier study, our results show that after excitation of the 13-cis form it… CONTINUE READING