Ultrafast folding kinetics and cooperativity of villin headpiece in single-molecule force spectroscopy

@inproceedings{oldka2013UltrafastFK,
  title={Ultrafast folding kinetics and cooperativity of villin headpiece in single-molecule force spectroscopy},
  author={Gabriel Žold{\'a}ka and Johannes Stiglera and Benjamin Pelza and Hongbin Lib and Matthias Riefa},
  year={2013}
}
  • Gabriel Žoldáka, Johannes Stiglera, +2 authors Matthias Riefa
  • Published 2013
In this study we expand the accessible dynamic range of singlemolecule force spectroscopy by optical tweezers to the microsecond range by fast sampling. We are able to investigate a single molecule for up to 15 min and with 300-kHz bandwidth as the protein undergoes tens of millions of folding/unfolding transitions. Using equilibrium analysis and autocorrelation analysis of the time traces, the full energetics as well as real-time kinetics of the ultrafast folding of villin headpiece 35 and a… CONTINUE READING