Ultra sensitive measurement of endogenous epinephrine and norepinephrine in human plasma by semi-automated SPE-LC-MS/MS.

@article{Zhang2012UltraSM,
  title={Ultra sensitive measurement of endogenous epinephrine and norepinephrine in human plasma by semi-automated SPE-LC-MS/MS.},
  author={Guodong Zhang and Yizhong Zhang and Chengjie Ji and Thomas Mcdonald and Justin Walton and Elizabeth A Groeber and Rick C Steenwyk and Zhaosheng Lin},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2012},
  volume={895-896},
  pages={186-90}
}
Measurement of endogenous epinephrine (E) and norepinephrine (NE) in human plasma is very challenging due to lower endogenous concentrations as compared with animal plasma. An LC-MS/MS in combination with alumina-based SPE and derivatization procedure was validated for the measurement of E and NE in human plasma with acceptable intra-day and inter-day accuracy and precision. Sample was extracted with semi-automated alumina 96-well solid phase extraction (SPE) cartridge. The resulting eluent was… CONTINUE READING