Ultra-rapid freezing of human multipronuclear zygotes using electron microscope grids.

@article{Park2000UltrarapidFO,
  title={Ultra-rapid freezing of human multipronuclear zygotes using electron microscope grids.},
  author={Sejin Park and Eun Young Kim and Ji Hwan Oh and Hyo Kyung Nam and Kyu Seung Lee and Stella Y. Park and Eun Mi Park and San Hyun Yoon and Kil Saeng Chung and Jin Ho Lim},
  journal={Human reproduction},
  year={2000},
  volume={15 8},
  pages={1787-90}
}
Developmental capacity of human multipronuclear (PN) zygotes cryopreserved using an ultra-rapid freezing method and electron microscope (EM) grids was studied. Multipronuclear zygotes obtained from a human IVF programme were used as an alternative to normal 2PN zygotes; they were divided into 3PN or >or =4PN zygotes and their in-vitro development and cryo-injury were compared according to PN number. EFS30, which consisted of 30% ethylene glycol, 18% Ficoll, 0. 5 mol/l sucrose and 10% fetal… CONTINUE READING