Ultra-high-resolution scanning electron microscopy of mitochondria and sarcoplasmic reticulum arrangement in human red, white, and intermediate muscle fibers.

@article{Ogata1997UltrahighresolutionSE,
  title={Ultra-high-resolution scanning electron microscopy of mitochondria and sarcoplasmic reticulum arrangement in human red, white, and intermediate muscle fibers.},
  author={Toshiro Ogata and Yuichi Yamasaki},
  journal={The Anatomical record},
  year={1997},
  volume={248 2},
  pages={214-23}
}
BACKGROUND Human skeletal muscle fibers are the red, white, and intermediate fibers. They differ in their mitochondrial structure and enzyme activity. Scanning electron microscopy (SEM) was used on specially prepared specimens to determine the distinctive features of mitochondria and sarcoplasmic reticulum (SR) in each fiber type. METHODS Specimens of human limb muscles were glutaraldehyde fixed, frozen, fractured, and macerated by the aldehyde-osmium-DMSO-osmium procedure to expose large… CONTINUE READING
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