Ultra-deep sequencing enables high-fidelity recovery of biodiversity for bulk arthropod samples without PCR amplification

@inproceedings{Zhou2013UltradeepSE,
  title={Ultra-deep sequencing enables high-fidelity recovery of biodiversity for bulk arthropod samples without PCR amplification},
  author={Xin Zhou and Yiyuan Li and Shanlin Liu and Qing Yang and Xu Su and Lili Zhou and Min Tang and Ribei Fu and Jiguang Li and Quanfei Huang},
  booktitle={GigaScience},
  year={2013}
}
BACKGROUND Next-generation-sequencing (NGS) technologies combined with a classic DNA barcoding approach have enabled fast and credible measurement for biodiversity of mixed environmental samples. However, the PCR amplification involved in nearly all existing NGS protocols inevitably introduces taxonomic biases. In the present study, we developed new Illumina pipelines without PCR amplifications to analyze terrestrial arthropod communities. RESULTS Mitochondrial enrichment directly followed by… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 19 times over the past 90 days. VIEW TWEETS