Ultra-deep sequencing confirms immunohistochemistry as a highly sensitive and specific method for detecting BRAF V600E mutations in colorectal carcinoma

@article{Roessle2013UltradeepSC,
  title={Ultra-deep sequencing confirms immunohistochemistry as a highly sensitive and specific method for detecting BRAF V600E mutations in colorectal carcinoma},
  author={Matthias Roessle and Mich{\`e}le Sigg and Jan H. R{\"u}schoff and Peter J. Wild and Holger Moch and Achim Weber and Markus P. Rechsteiner},
  journal={Virchows Archiv},
  year={2013},
  volume={463},
  pages={623-631}
}
The activating BRAF V600 mutation is a well-established negative prognostic biomarker in metastatic colorectal carcinoma (CRC). A recently developed monoclonal mouse antibody (clone VE1) has been shown to detect reliably BRAF V600E mutated protein by immunohistochemistry (IHC). In this study, we aimed to compare the detection of BRAF V600E mutations by IHC, Sanger sequencing (SaS), and ultra-deep sequencing (UDS) in CRC. VE1-IHC was established in a cohort of 68 KRAS wild-type CRCs. The VE1-IHC… CONTINUE READING