Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo.

@article{Engelbrecht2008UltracompactFT,
  title={Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo.},
  author={Christoph J Engelbrecht and Richard S. Johnston and Eric J. Seibel and Fritjof Helmchen},
  journal={Optics express},
  year={2008},
  volume={16 8},
  pages={5556-64}
}
We present a small, lightweight two-photon fiberscope and demonstrate its suitability for functional imaging in the intact brain. Our device consists of a hollow-core photonic crystal fiber for efficient delivery of near-IR femtosecond laser pulses, a spiral fiber-scanner for resonant beam steering, and a gradient-index lens system for fluorescence excitation, dichroic beam splitting, and signal collection. Fluorescence light is remotely detected using a standard photomultiplier tube. All… CONTINUE READING