Ubiquitous Sialometabolism Present among Oral Fusobacteria

@article{Yoneda2014UbiquitousSP,
  title={Ubiquitous Sialometabolism Present among Oral Fusobacteria},
  author={Saori Yoneda and Brandon Loeser and J. Feng and John J. Dmytryk and Fengxia Qi and Justin Merritt},
  journal={PLoS ONE},
  year={2014},
  volume={9}
}
Fusobacterium nucleatum is a ubiquitous member of the human oral flora and is associated with the development of periodontitis and a variety of other types of polymicrobial infections of the mucosa. In the oral cavity, this species is one of the few that is prevalent in both healthy and diseased subgingival plaque. Using microarray analysis, we examined the transcriptional response of F. nucleatum subspecies nucleatum to whole blood in order to identify some of the genetic responses that might… 

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References

SHOWING 1-10 OF 52 REFERENCES
Autoaggregation Response of Fusobacterium nucleatum
TLDR
It appears that autoaggregation activates a genetic program that may be utilized for growth in a high cell density environment, such as the oral biofilm.
Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum
TLDR
This review covers what is known to date about F. nucleatum in general, such as taxonomy and biology, with special emphasis on its pathogenic potential, and its possible relationship to otherperiodontal bacteria in the development of periodontal diseases and the possible roles played by OMPs.
Fusobacterium nucleatum
TLDR
An intriguing picture emerges that paints F. nucleatum as both conferring beneficial as well as detrimental effects on host cells; and it is suggested that the ultimate effects of F.ucleatum infection in the gut are a consequence of the microbes with which this species aggregates.
Sialic acid, periodontal pathogens and Tannerella forsythia: stick around and enjoy the feast!
TLDR
A review of periodontal bacteria have a range of sialic acid uptake and utilization systems while others may also use sIALic acid as a cloaking device on their surface to mimic host and avoid immune recognition.
Fusobacterium nucleatum Infection of Colonic Cells Stimulates MUC2 Mucin and Tumor Necrosis Factor Alpha
TLDR
It is demonstrated that highly invasive F. nucleatum isolates derived from the inflamed guts of Crohn's disease patients evoked significantly greater MUC2 and tumor necrosis factor alpha (TNF-α) gene expression than minimally invasive strains isolated from the noninflamed gut in human colonic epithelial cells and in a rat ligated colonic loop model of infection.
Application of rpoB and Zinc Protease Gene for Use in Molecular Discrimination of Fusobacterium nucleatum Subspecies
TLDR
The results suggest that rpoB and the zinc protease gene are efficient targets for the discrimination and taxonomic analysis of the subspecies of F. nucleatum.
Complementation of the fadA Mutation in Fusobacterium nucleatum Demonstrates that the Surface-Exposed Adhesin Promotes Cellular Invasion and Placental Colonization
TLDR
FadA plays an important role in F. nucleatum colonization in vivo and may be a therapeutic target for preventing F.ucleatum colonization of the host, according to previous studies.
Invasive potential of gut mucosa‐derived fusobacterium nucleatum positively correlates with IBD status of the host
TLDR
This study indicates that colonization of the intestinal mucosa by highly invasive strains of F. nucleatum may be a useful biomarker for gastrointestinal disease.
Diversity of Microbial Sialic Acid Metabolism
TLDR
Sialic acids are structurally unique nine-carbon keto sugars occupying the interface between the host and commensal or pathogenic microorganisms, and are a host molecule to be copied, eaten, and interpreted by diverse metabolic machinery in all major groups of mammalian pathogens and Commensals.
The Fusobacterium nucleatum outer membrane protein RadD is an arginine‐inhibitable adhesin required for inter‐species adherence and the structured architecture of multispecies biofilm
TLDR
Findings indicate that radD is responsible for arginine‐inhibitable adherence of F.’nucleatum and provides definitive molecular evidence that F.Nucleatum adhesins play a vital role in inter‐species adherence and multispecies biofilm formation.
...
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