Ubiquitin fusion constructs allow the expression and purification of multi-KOW domain complexes of the Saccharomyces cerevisiae transcription elongation factor Spt4/5.

@article{Blythe2014UbiquitinFC,
  title={Ubiquitin fusion constructs allow the expression and purification of multi-KOW domain complexes of the Saccharomyces cerevisiae transcription elongation factor Spt4/5.},
  author={Amanda Blythe and Sanjika Dias Gunasekara and James Walshe and Joel P Mackay and Grant A. Hartzog and Alice Vrielink},
  journal={Protein expression and purification},
  year={2014},
  volume={100},
  pages={
          54-60
        }
}
Spt4/5 is a hetero-dimeric transcription elongation factor that can both inhibit and promote transcription elongation by RNA polymerase II (RNAPII). However, Spt4/5's mechanism of action remains elusive. Spt5 is an essential protein and the only universally-conserved RNAP-associated transcription elongation factor. The protein contains multiple Kyrpides, Ouzounis and Woese (KOW) domains. These domains, in other proteins, are thought to bind RNA although there is little direct evidence in the… CONTINUE READING
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