Ubiquitin C-Terminal Hydrolase-Activity Is Involved in Sperm Acrosomal Function and Anti-Polyspermy Defense During Porcine Fertilization1

@inproceedings{Yi2007UbiquitinCH,
  title={Ubiquitin C-Terminal Hydrolase-Activity Is Involved in Sperm Acrosomal Function and Anti-Polyspermy Defense During Porcine Fertilization1},
  author={Young-Joo Yi and Gaurishankar Manandhar and Miriam Sutovsky and Rongfeng Li and Věra Jon{\'a}kov{\'a} and Richard J. Oko and Chang-Sik Park and Randall S Prather and Peter Sutovsky},
  booktitle={Biology of reproduction},
  year={2007}
}
Abstract The 26S proteasome, which is a multi-subunit protease with specificity for substrate proteins that are postranslationally modified by ubiquitination, has been implicated in acrosomal function and sperm-zona pellucida (ZP) penetration during mammalian fertilization. Ubiquitin C-terminal hydrolases (UCHs) are responsible for the removal of polyubiquitin chains during substrate priming for proteasomal proteolysis. The inhibition of deubiquitination increases the rate of proteasomal… Expand
Role of Ubiquitin C-Terminal Hydrolase-L1 in Antipolyspermy Defense of Mammalian Oocytes1
TLDR
The dynamics of cortical granules in UCHL1-inhibited oocytes were analyzed; both migration of CGs toward the cortex during oocyte maturation and fertilization-induced extrusion ofCGs were impaired, indicating that antipolyspermy defense in bovine oocytes may rely on UCHL 1-controlled functioning of CGS. Expand
Ubiquitin-activating enzyme (UBA1) is required for sperm capacitation, acrosomal exocytosis and sperm-egg coat penetration during porcine fertilization.
TLDR
It appears that de novo protein ubiquitination involving UBA1 contributes to sperm capacitation and acrosomal function during fertilization. Expand
Sperm proteasome and fertilization.
TLDR
The studies on sperm proteasome indicate that animal fertilization is controlled in part by a unique, gamete associated, extracellular UPS, and diagnostic and therapeutic applications could emerge in human reproductive medicine. Expand
Inhibition of 19S proteasomal regulatory complex subunit PSMD8 increases polyspermy during porcine fertilization in vitro.
TLDR
The present study examined the role of PSMD8, an essential non-ATPase subunit of the 19S complex, in sperm-ZP penetration during porcine fertilization in vitro (IVF), and observed that the sperm acrosome-borne proteasomes become exposed onto the sperm surface following the acrosomal exocytosis. Expand
Ubiquitin Carboxy-Terminal HydrolaseL3 Correlates with Human Sperm Count, Motility and Fertilization
TLDR
The level and activity of UCHL3 were positively correlated with sperm count, concentration and motility, and correlations were not manifested in intra-cytoplasmic sperm injection (ICSI). Expand
Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization
TLDR
The 19S regulatory complex subunit PSMD4 is involved in the sperm-ZP penetration during fertilization, and the recognition of substrates on the ZP by the 19S proteasomal regulatory complex is essential for the success of porcine/mammalian fertilization in vitro. Expand
Protein deubiquitination during oocyte maturation influences sperm function during fertilisation, antipolyspermy defense and embryo development.
TLDR
It is concluded that DUB inhibitors alter porcine oocyte maturation, fertilisation and preimplantation embryo development by regulating the turnover of oocyte proteins and mono-ubiquitin regeneration, the DUBs may promote the acquisition of developmental competence during oocytes maturation. Expand
Sperm-Surface ATP in Boar Spermatozoa is Required for Fertilization: Relevance to Sperm Proteasomal Function
TLDR
The present data provide an important missing piece of evidence in support of the ssATP-dependent, proteasomal-proteolytic model of sperm-ZP interactions. Expand
Essential role of ubiquitin C‐terminal hydrolases UCHL1 and UCHL3 in mammalian oocyte maturation
TLDR
It is concluded that the activity of oocyte UCHs contributes to oocyte maturation by regulating the oocyte cortex and meiotic spindle. Expand
The sperm proteasome during sperm capacitation and fertilization.
TLDR
Evidence is provided for the involvement of the 26S proteasome in multiple steps of animal and human fertilization, offering a novel model of sperm-egg coat interactions, and identifying a range of potential new sperm quality markers and contraceptive targets. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 77 REFERENCES
Localization of ubiquitin C-terminal hydrolase L1 in mouse ova and its function in the plasma membrane to block polyspermy.
TLDR
Results may identify UCH-L1 as a candidate for a sperm-oocyte interactive binding or fusion protein on the plasma membrane that functions during the block to polyspermy in mouse oocytes. Expand
Developmental Regulation of Ubiquitin C-Terminal Hydrolase Isozyme Expression During Spermatogenesis in Mice
TLDR
UCH-L1 and UCH-L3 have distinct functions during spermatogenesis, namely, that UCH- l1 may act during mitotic proliferation of sperMatogonial stem cells whereas UCH- L3 may function in the meiotic differentiation of s permatocytes into sper matids. Expand
Ubiquitin‐dependent proteolysis in mammalian spermatogenesis, fertilization, and sperm quality control: Killing three birds with one stone
  • P. Sutovsky
  • Biology, Medicine
  • Microscopy research and technique
  • 2003
TLDR
Deciphering and eventually manipulating the ubiquitin‐dependent proteolysis in the reproductive system could allow us to redirect the mode of mtDNA inheritance after cloning and ooplasmic transplantation, provide germ line therapy in some cases of male infertility, develop new contraceptives, manage polyspermia during in vitro fertilization, and establish objective markers for infertility diagnostics, semen evaluation, and prediction of future fertility. Expand
Extracellular ubiquitination and proteasome-mediated degradation of the ascidian sperm receptor
  • H. Sawada, N. Sakai, +6 authors H. Yokosawa
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2002
TLDR
Results indicate that the ubiquitin–proteasome system is responsible for extracellular degradation of the sperm receptor HrVC70 and, consequently, for sperm penetration of the vitelline coat during fertilization. Expand
PAWP, a Sperm-specific WW Domain-binding Protein, Promotes Meiotic Resumption and Pronuclear Development during Fertilization*♦
TLDR
A group I WW domain module signal transduction event within the fertilized egg that appears compulsory for meiotic resumption and pronuclear development during egg activation is uncovered and provides compelling evidence that a PPXY motif of sperm-contributed PAWP can trigger these events. Expand
Extracellular localization of proteasomes in human sperm
TLDR
Evidence is presented indicating that the proteasome has an extracellular localization, on the plasma membrane of the sperm head, and plays an important role during the acrosome reaction. Expand
Proteasomal Interference Prevents Zona Pellucida Penetration and Fertilization in Mammals1
TLDR
Experimental evidence is provided demonstrating proteasome involvement in the penetration of mammalian zona pellucida (ZP) and implicating the ubiquitin-proteasome pathway in mammalian fertilization and zona penetration, which opens a new line of acrosome/ZP research. Expand
Two closely related ubiquitin C-terminal hydrolase isozymes function as reciprocal modulators of germ cell apoptosis in cryptorchid testis.
TLDR
Analysis of the function of two closely related members of the ubiquitin C-terminal hydrolase (UCH) family in testicular germ cell apoptosis experimentally induced by cryptorchidism demonstrates that UCH-L1 and UCH- L3 function differentially to regulate the cellular levels of anti-apoptotic, prosurvival, and apoptotic proteins during testicular Germ cell apoptoses. Expand
Participation of the sperm proteasome in human fertilization.
TLDR
The human sperm proteasome is involved in the exocytosis of the acrosome, perhaps in events upstream of the plateau phase of the Ca(2+) influx. Expand
Localization and roles in fertilization of sperm proteasomes in the ascidian Halocynthia roretzi
TLDR
Results indicate that H. roretzi sperm has the membrane‐associated proteasome on its head, which is activated upon sperm activation, and that sperm proteasomesome plays an essential role in H.roretzi fertilization. Expand
...
1
2
3
4
5
...