UV microspectrophotometry of mitochondrial responses to extracellular glucose in cultured ascites tumor cells.

@article{Ritter1971UVMO,
  title={UV microspectrophotometry of mitochondrial responses to extracellular glucose in cultured ascites tumor cells.},
  author={C. Ritter and B. Thorell},
  journal={Experimental cell research},
  year={1971},
  volume={65 1},
  pages={
          233-9
        }
}
Summary Extracellular glucose addition to cultured ascites cells is followed by a decrease in UV absorption of intracellular mitochondria. Dicumarol also produces a decrease in such UV absorption and, following a maximum effect, addition of glucose is ineffective. Deoxyglucose reversibly inhibits the effect of glucose. It is proposed that the mitochondrial UV absorbing material, which is lost following extracellular glucose addition, is adenine nucleotide and that the adenine nucleotide loss is… Expand
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TLDR
It is proposed that the main factor responsible for the large decrease in 265 mµ absorption is adenine nucleotide loss, which is more than would be expected based on the increase in absorption seen at 330-340mµ. Expand
Theoretical phosphorylation rates after addition of a small amount of glucose to intact ascites tumor cells.
TLDR
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TLDR
Microspectrographic techniques have been developed which permit measurements of specific absorptions of about a per cent in the long-wave ultraviolet and visible wavelength region from intact, living-cell areas of 1.5 μ in diameter, and showed that the metabolic state of the respiratory system of small parts in the single, intact mammalian cell could be analyzed. Expand
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Abstract For brain phosphofructokinase at pH 8, with noninhibitory levels of adenosine triphosphate, the Michaelis constants for ATP (0.1 mm) and fructose-6-P (0.04 mm) are each independent of theExpand
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TLDR
The binding properties of purified sheep heart phosphofructokinase were studied with a gel filtration technique and were compared with those of enzyme desensitized to allosteric control by photooxidation to show the effect of different enzyme effectors on the binding of the above mentioned substrates. Expand
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