UV-irradiation induces oxidative damage to mitochondrial DNA primarily through hydrogen peroxide: analysis of 8-oxodGuo by HPLC.

@article{Takai2006UVirradiationIO,
  title={UV-irradiation induces oxidative damage to mitochondrial DNA primarily through hydrogen peroxide: analysis of 8-oxodGuo by HPLC.},
  author={Daisaku Takai and Sang-Hee Ko Park and Yasunari Takada and Shizuko Ichinose and Masanobu Kitagawa and Makoto Akashi},
  journal={Free radical research},
  year={2006},
  volume={40 11},
  pages={
          1138-48
        }
}
Roles of reactive oxygen species (ROS) in damage to mitochondrial DNA (mtDNA) following ultraviolet (UV)-irradiation were investigated in the human hepatoma cell line SK-HEP-1. We altered the intracellular status of ROS by the overexpression of manganese superoxide dismutase (MnSOD) and/or catalase. Using HPLC, we analyzed 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), known as a marker of damage to DNA molecules. UV-irradiation resulted in the accumulation of 8-oxodGuo in these cells. The… CONTINUE READING