UV extinction coef ® cients for nucleoside-5 ¢-monophosphates and unpaired DNA and RNA

Ultraviolet absorption provides the nearly universal basis for determining concentrations of nucleic acids. Values for the UV extinction coef®cients of DNA and RNA rely on the mononucleotide values determined 30±50 years ago. We show that nearly all of the previously published extinction coef®cients for the nucleoside-5¢-monophosphates are too large, and in… CONTINUE READING