UV cross-linking of interacting RNA and protein in cultured cells.

Abstract

RNA-protein interactions play indispensable roles in the regulation of cellular functions. Biochemical characterization of these complexes is often done by immunoprecipitation (IP) of RNA-binding proteins (RBPs) followed by identification of co-immunoprecipitated RNAs. This protocol couples ultraviolet (UV) irradiation with IP to determine whether a specific protein interacts directly with a specific RNA in living cells.

DOI: 10.1016/B978-0-12-420120-0.00004-9

Cite this paper

@article{Sei2014UVCO, title={UV cross-linking of interacting RNA and protein in cultured cells.}, author={Emi Sei and Nicholas K. Conrad}, journal={Methods in enzymology}, year={2014}, volume={539}, pages={53-66} }