UV-B Irradiated Cell Lines Execute Programmed Cell Death in Various Forms

@article{Hagenhofer1998UVBIC,
  title={UV-B Irradiated Cell Lines Execute Programmed Cell Death in Various Forms},
  author={M. Hagenhofer and H. Germaier and Christine Hohenadl and Peter Rohwer and Joachim. R. Kalden and Martin Herrmann},
  journal={Apoptosis},
  year={1998},
  volume={3},
  pages={123-132}
}
We investigated changes typical for apoptosis in various cell lines after UV-B irradiation. Using established methods for detection of apoptosis we demonstrate changes of cellular morphology, phosphatidylserine (PS) exposure, ollgonucleosomal DNA fragmentation and generation of hypochrome nuclei. To isolated high-molecular-weight (hmwt) DNA fragments we engaged a new method avoiding pulse field gel electrophoresis. Most UV-B irradiated cell lines showed oligonucleosomal DNA fragmentation… CONTINUE READING